SUT

School of Chemistry  

Suranaree University of Technology     

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Assistant Professor Dr. James R. Ketudat-Cairns
ผู้ช่วยศาสตราจารย์ ดร. เจมส์ เกตุทัต-คาร์นส์

Biochemistry

Tel. 044-224304, 044-223294    Fax. 044-224-185
e-mail: cairns@sut.ac.th

 

Research Interest
     Dr. James Ketudat Cairns basic interest is in protein structure-function relationships. Current model systems are the glycosyl hydrolases and metabolic enzymes defects found in inborn errors of metabolism. The main thrust of the group at SUT is the study of glycosyl hydrolase structure, biochemical activity and biological function. Beta-glucosidase, beta-galactosidase, and chitinase enzymes are currently being investigated in these regards. One of the main projects is on the functional geomics of rice b-glycosidases: the structure and biological function of beta-glucosidase and beta-galactosidase in rice germination, growth, and environmental responses. Perhaps the most fundamental of the projects in the group is the isolation and activity characterization of beta-glucosidases, analysis of their sequences, and molecular modeling of their enzyme-substrate interactions.

Degrees:
1986    BSc (Biology with Chemistry and Mathematics Minors) University of Puget Sound, USA
1993    PhD (Biology) University of California, San Diego, USA

Publications:

Journal Articles:

  1. Opassiri R, Hua Y, Wara-Aswapati O, Akiyama T, Svasti J, Esen A, and Ketudat Cairns JR. 2004. b -Glucosidase, exo-b-glucanase and pyridoxine transglucosylase activities of rice BGlu1. Biochemical Journal 379, 125-131.
    DOI: 10.1042/BJ20031485.

  2. Opassiri R, Ketudat Cairns JR, Akiyama T, Wara-Aswapati O, Svasti J, and Esen A. 2003. Characterization of a rice b-glucosidase genes highly expressed in flower and germinating shoot Plant Science 165, 627-638.

  3. Kaomek M, Mizuno K, Fujimua T, Sriyotha P, Ketudat Cairns JR. 2003. Cloning, Expression and Characterization of an Anti-Fungal Chitinase from Leucaena leucocephala de Wit. Bioscience, Biotechnology and Biochemistry 67, 667-676.

  4. Champattanachai V, Ketudat Cairns, JR, Shotelersuk V, Kerratichamreon S, Sawangareetrakul P, Srisomsap C, Kaewpaluek V, Svasti J. 2003. Novel Mutations in a Thai patient with methylmalonic acidemia. Molecular Genetics and Metabolism 79, 300-302.

  5. Svasti S, Yodsowon B, Sriphanich R, Winichagoon P, Boonkhan P, Suwanban T, Sawangareetrakul P, Srisomsap C, Ketudat-Cairns J, Svasti J, Fucharoen S. 2001. Association of Hb Hope [b136(H14)Gly à Asp] and Hb H Disease. Hemoglobin 25, 429-435.

  6. Ketudat Cairns JR, Champattanchai V, Srisosap C, Thiede B, Wittman-Liebold B, Svasti J. 2000. Sequence and Expression of Thai Rosewood b-Glucosidase/b-Fucosidase, a Family 1 Glycosyl Hydrolase Glycoprotein. Journal of Biochemistry 128, 999-1008.

  7. Ketudat Cairns JR, Chantarangsee M, Chaiwangrad S, Phawong J. 1999. Primary Structure-Based Screening for Glycosyl Hydrolases in Thai Plants. Thai Journal of Biotechnology 1, 20-30.

  8. Svasti J, Srisomsap C, Surarit R, Techasakul S, and Ketudat-Cairns JR. 1998. Characterization of a Novel Rotenoid-b-Glucosidase and its Natural Substrate from Thai Rosewood. Journal of Pure and Applied Chemistry 70 (11), http://www.iupac.org/symposia/proceedings/phuket97/svasti.html.

  9. Cairns JR, Price PA. 1994. Direct Demonstration That the Vitamin K-Dependent Bone Gla Protein Is Incompletely g-Carboxylated in Humans. Journal of Bone and Mineral Research 9, 1989-1997.

  10. Cairns JR, Williamson MK, Price PA. 1991. Direct Identification of g-Carboxyglutamic Acid in the Sequencing of Vitamin K-Dependent Proteins. Analytical Biochemistry 199, 93-97.

Selected Recent Presentations:

  1. Opassiri, R. and Ketudat Cairns, J.R. (2004) Exo-beta-glucanase and transglycosylase activities of rice BGlu1 beta-glucosidase. (oral presentation) 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p. 27

  2. Chantarangsee, M. and Ketudat Cairns, J.R. (2004) Molecular cloning and expression of rice b-galactosidase in E.coli BL21(DE3). (poster presentation) 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p.80

  3. Metheenukul, P., Ketudat-Cairns, J.R., an Fujimura, T. (2004) Recombinant expresion of Thai rosewood b-glucosidase in E. coli. 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p.85

  4. Chuenchor, W., Opassiri, R., Ketudat Cairns, J.R. (2004) Recombinant expression and preliminary crystallization of rice BGlu1 beta-glucosidase. 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p. 98

  5. Mothong, N., Chuankhayan, P., Toonkook, P., and Ketudat Cairns, J.R.. (2004) Cloning and sequencing of a full-length cDNA encoding a new b-glucosidase rom Dalbergia nigrescens Kurz. 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p. 68

  6. Chuankhayan, P., Ketudat-Cairns, J., and Hua, Y. (2004) The purification of isoflavonoid glycoside substrates from Dalbergia nigrescens Kurz. 15th Annual Meeting of the Thai Society for Biotechnology, Chiang Mai, Thailand, 2-6 February, 2004. Abstracts p.70

  7. Ketudat Cairns JR, Opassiri R, Chantarangsee M, Chuenchor W, Akiyama T, Esen J, Svasti J, Wara-Aswapati O. 2003. Investigation of Glycosyl Hydrolase Family 1 and 35 Genes in Rice. Rice Biotechnology 2003/ BioThailand 2003. PEACH Pattaya, Thailand, July 18, 2003. O-RICE-02. Proceedings pgs. 23-28.

  8. Opassiri R., Ketudat Cairns JR, Akiyama T, Wara-Aswapati O, Svasti J, Esen A. 2003. cDNA cloning, recombinant protein expression, and functional characterization of a rice b-glucosidase highly expressed in flower and germinating shoot. Rice Biotechnology 2003/ BioThailand 2003. PEACH Pattaya, Thailand, July 18, 2003. P-RICE-09, Proceedings pg. 81.

  9. Svasti J, Ketudat-Cairns J, Srisomsap C, Surarit R, Techasakul S, and Toonkool P. 2003. Structure and properties of beta-glucosidases from Thai Plants. International Symposium on Protein Structure Function. Karachi, Pakistan, Jan., 2003.

  10. Svasti J, Srisomsap C, Surarit R, Ketudat-Cairns J, Techasakul S. Catalytic action of Thai plant b-glucosidases. Fifth Asia-Pacific International Molecular Biology Network, Shanghai, November, 2002.

  11. Ketudat Cairns JR, Sucharoen S, Champattanachai V, Keeratichamroen S, Surarit R, Srisomsap C, Wasant P, Svasti J. Identification of a new mutation and polymorphisms in a Thai case of Hurler syndrome. 4th HUGO Pacific Meeting and 5th Asia-Pacific Conference on Human Genetics. Pattaya, Thailand, October 27-30, 2002. Abstract SG-18.

  12. Champattanachai, V., Keeratichamroen S, Sawangareetrakul, Ketudat-Cairns JR, Srisomsap C, Shotelersuk V, Svasti J. 2002. Identification of two novel mutations in the methylmalonyl CoA mutase from a Thai patient with acidemia. 4th HUGO Pacific Meeting and 5th Asia-Pacific Conference on Human Genetics. Pattaya, Thailand, October 27-30, 2002. Abs. IM-05

  13. Ngiwsara L, Srisomsap C, Fucharoen P, Fucharoen S, Sawangareetrakul P, Ketudat-Cairns JR, Svasti J. 2002. Hemoglobin Koaira [b146(HC3)His > Gln], a high oxygen affinity, observed in Thailand. 4th HUGO Pacific Meeting and 5th Asia-Pacific Conference on Human Genetics. Pattaya, Thailand, October 27-30, 2002. Abs. TH-14.

  14. Ngiwsara L, Srisomsap C, Fucharoen P, Fucharoen S, Ketudat-Cairns JR, Svasti J. Characterization of hemoglobin Hekinan [a27Glu(GAG) > Asp(GAT) in Thailand. 4th HUGO Pacific Meeting and 5th Asia-Pacific Conference on Human Genetics. Pattaya, Thailand, October 27-30, 2002. TH-15

  15. Opassiri R, Ketudat-Cairns JR, Wara-Aswapati O, Akiyama T, Svasti J, Esen A. Structure and Expression of b-glucosidase genes in rice (Oryza sativa L.). Plant Biology 2002. The Annual Meeting of the American Society of Plant Biologists, Denver CO, USA Aug 3-7, 2002. Abstracts pg 45 Session 27 # 62.

  16. Ketudat Cairns JR, Champattanachai V, Chuankhayan P, Srisomsap C, Boonpuan K, Sullivan PA, Svasti J. “Dalbergia beta-glucosidases: structure and functional differences.” 15th Protein Symposium, 28 July-1 August, 2001, Philadelphia, PA, USA. Abstract number 163. Protein Science 10 (Suppl. 2), 94-95.

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